Coding

Part:BBa_J72006:Design

Designed by: John Anderson   Group: Anderson Lab   (2008-07-16)


{M.BamHI-2} BamHI methyltransferase in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

PCR ca1149F/ca1142R on Bacillus amyloliquefaciens genomic DNA  (BglII/XhoI)
Sub into pBca9145-Bca1144#5                                    (BglII/XhoI, L)
Product is pBca9145-Bca1149
----
ca1149F	Forward BglII for M.BamHI
ggtgtAGATCTatgcgAttCttCtctgtCtttgacattg
ca1142R	Reverse Biobricking of M.bamHI
gcaaaCTCGAGttaGGATCCTCATTCcAGACTTAATGAAATTTGGTC

References